Diurnal Effect of Selenium Supplementation on Adult Female Wistar Rats made Hypothyroid by Methimazole

MJ Adeniyi* and FO Agoreyo

DOI: 10.26717/BJSTR.2017.01.000175

Received: June 30, 2017;   Published: July 07, 2017

To view the Full Article   Peer-reviewed Article PDF

Abstract

Diurnal variation in gastric emptying is known to affect the bioavailability of ingest. The present study sought to understand the effect of consumption of selenium in daytime or nighttime on some clinical features of hypothyroidism in female wistar rats. 36 adult female wistar rats weighing 120-150g were randomly divided into 6 groups: vehicle-treated, methimazole, daytime – selenium, nighttime-selenium, methimazole+ daytime-selenium and methimazole+ nighttime selenium groups. Hypothyroidism was induced through oral administration of 1.35mg/kg body weight of methimazole. 7mg/ml of Selenomethionine was administered through drinking water for two weeks. When compared with vehicletreated group, methimazole administration caused a significant (P<0.05) decrease in caudal cold tolerance (CCT) and Body Temperature (BT). Rats administered selenium during day-time showed a significantly higher (P<0.05) plasma glucose and decreased CCT. Nighttime selenium administration to hypothyroid rats resulted in a significant decrease (P<0.05) in plasma glucose. There was an insignificant change in CCT and BT in hypothyroid rats administered selenium in day-time. In conclusion, the study showed that daytime selenium consumption may exert more positive influence on hypothyroidism than nighttime selenium consumption.

Keywords: Methimazole; Hypothyroidism; Caudal cold tolerance; Body temperature; Selenium

Abbreviations: CCT: Caudal Cold Tolerance; BT: Body Temperature; NIH: National Institute of Health; VEH: Vehicle-Treated group; DTSE: Day- Time Selenium group; NTSE: Night-Time selenium groups

Abstract | Introduction | Materials and Methods | Methods | Results | Discussion and Conclusion | References |