Research ArticleOpen Access

Effect of Nd: YAG Low Level Laser Therapy on human periodontal ligament cells: A preliminary in-vitro study

Volume 2 - Issue 4

**Ioannis D Prevezanos*, Ioannis K Karoussis, Andreas S Gkogkos, Kleopatra E Marcopoulou, Kyriaki Kyriakidou, Anastasia Chernysheva and Ioannis A Vrotsos**

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- Department of Periodontology, National and Kapodistrian University of Athens, Greece
*Corresponding author: Ioannis D Prevezanos, School of Dentistry, Department of Periodontology, National and Kapodistrian University of Athens, 2 Thivon str, Goudi, 11517, Athens, Greece

Received: February 13, 2018; Published: February 26, 2018

DOI: 10.26717/BJSTR.2018.02.000797

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Abstract

Aim: To investigate the effect of Nd:YAG (1064-nm) laser irradiation on human periodontal ligament cells, concerning proliferation, differentiation and certain growth factors secretion: vascular endothelial growth factor (VEGF), epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF).

Materials and Methods: Primary PDL cell cultures were irradiated with Nd: YAG laser (1064-nm), for 20, 40, 60 and 120 seconds (energy densities: 2.6-15.8J/cm²) and evaluated after 24 and 72 hours. Controls were not irradiated. The experiment was performed in duplicate. Data analyzed by Students t-test (p<0.05).

Results: Cell cultures irradiated for 40, 60 and 120 seconds revealed higher proliferation when compared to controls, 24 and 72 hours after irradiation. Statistical significance was reached for the group of 60s at 24h and for groups of 40s and 120s at 72h (p<0.05). Alkaline phosphatase (ALP) levels were also found increased for most of the irradiated cell cultures compared to controls. Statistical significance was reached for the 120s cultures at 24h as well as for the 20s at 72h (p<0.05). Growth factors’ levels for irradiated cultures and controls were arbitrary during the experiment.

Conclusion: Within the limitations of this study, Nd:YAG (1064-nm) LLLT with the energy densities applied, increased PDL cells proliferation 24 and 72 hours after irradiation. Further studies need to confirm these results and to reveal any influence on ALP levels and growth factors secretion.

Keywords: Differentiation, Growth Factors; LLLT; PDL cells; Proliferation

Abbreviations: PDL: Periodontal Ligament; LLLT: Low Level Leaser Therapy; NDYAG: Neodymium-Doped Yttrium Aluminium Garnet; VEGF: Vascular Endothelial Growth Factor; EGF: Epidermal Growth Factor; BFGF: Basic Fibroblast Growth Factor; ALP: Alkaline Phosphatase