Research ArticleOpen Access

Major Compounds from Ocimum Basilicum l. and their Antimicrobial Activity against Methicillin-Resistant Staphylococcus Aureus

Volume 3 - Issue 3

**Thongchai Taechowisan*¹, Jakkrit Jantiya¹, Nutthapol Mungchukeatsakul¹, and Waya S Phutdhawong²**

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- ¹Department of Microbiology, Silpakorn University, Thailand
- ²Department of Chemistry, Silpakorn University, Thailand
*Corresponding author: Thongchai Taechowisan, Department of Microbiology, Faculty of Science, Silpakorn University, Nakorn Pathom 73000,Thailand

Received: March 09, 2018; Published: April 04, 2018

DOI: 10.26717/BJSTR.2018.03.000910

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Abstract

Sweet Basil (Ocimum basilicum Linn.) has a variety of therapeutic properties, including antimicrobial activity. This study aimed to determine the antibacterial activity of the major chemical components in the crude extract against methicillin resistant Staphylococcus aureus (MRSA) isolated from the clinical specimens. TLC and column chromatography resulted in the separation of two major compounds. On the basis of the spectral data, they were identified as linalool (1) and 1,8-cineole (2). Antibacterial activity of crude extract and isolated compounds was evalusted by disc diffusion, MIC and MBC against 9 multi-drug resistance S. aureus isolated from clinical specimens. The crude extract and isolated compounds exhibited an excellent antimicrobial activity at a dose level of 34.5 mg/disc for disc diffusion method. The crude extract and linalool showed higher activity against tested MRSA with MIC (<0.09 mg/ml) and MBC (≤ 0.09 to 0.38 mg/ml) than 1,8-cineole. The results suggest once again that the antimicrobial activity of the crude extract and major isolated compounds, linalool and 1,8-cineole are a resultant of the antibacterial property against MRSA.

Keywords: Antimicrobial Activity; Chemical Composition; Methicillin-Resistant Staphylococcus Aureus; Ocimum Basilicum L

Abbreviations: MRSA: Methicillin Resistant Staphylococcus Aureus; NMR: Nuclear Magnetic Resonance UV : Ultra Violet; TLC: Thin Layer Chromatography; BA: Blood Agar; MSA: Mannitol Salt Agar; MIC: Minimum Inhibitory Concentration; MBC: Minimum bactericidal concentration; PCR: Polymerase Chain Reaction