Fast and Accurate Electrochemical Measurement of Total Antioxidant Capacity as an Alternative to Spectrophotometrical Methods

Susana Rey¹, Esther Gómez¹, Henar Muñoz-Cimadevilla¹ and David Hevia*^1,2

Received: November 16, 2018;   Published: November 20, 2018

DOI: 10.26717/BJSTR.2018.11.002069

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Abstract

Total Antioxidant Capacity or TAC is an indicator of the sample ability to scavenge free radicals despite its complex composition. It has been measured in biological fluids as an inverse biomarker of oxidative stress, which has been related to disease. Classical spectrophotometric methods present some limitations including sample pretreatment required leading to long assay procedures, native pH alteration, low stability of some reagents, high detection limits, low sensitivity and sample’s colour interference. Various electrochemical techniques have raised as more precise alternatives that overcome most of the limitations in classical methodologies and have been gaining popularity specially for food and beverage analysis. In this mini review, an electrochemical measurement of antioxidant capacity with applications in vitro and in vivo is presented.

Keywords : Total Antioxidant Capacity; Electrochemical; Voltammetry; Biological samples; Oxidative stress

Abbreviations : TAC: Total Antioxidant Capacity; TEAC: Trolox Equivalent Antioxidant Capacity, GAE: Gallic Acid Equivalents; CEAC: Vitamin C Equivalents Antioxidant Capacity