Extraction Optimization for Phenols and Flavonoids
from Cultured Mycelia of Cordyceps Ophioglossoides
and Exploration of Bioactivities of its Aqueous and
Ethanol Extracts

Qinqin Xu; Zhao Zhao; Yisheng Sun; Ryan P Mackay; Yong-Quan Li

doi:10.26717/BJSTR.2018.11.002126

Research ArticleOpen Access

Extraction Optimization for Phenols and Flavonoids from Cultured Mycelia of Cordyceps Ophioglossoides and Exploration of Bioactivities of its Aqueous and Ethanol Extracts

Volume 11 - Issue 4

**Qinqin Xu^1,2, Zhao Zhao^1,3, Yisheng Sun^1,4, Ryan P Mackay² and Yong-Quan Li*¹**

Author Information Open or Close
- ¹Institute of Pharmaceutical Biotechnology& First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China
- ²Departments of Otolaryngology, Molecular and Cellular Physiology, Feist-Weiller Cancer Center, LSU Health Shreveport, 1501 Kings Highway, Shreveport, LA 71103, USA
- ³Centre of Laboratory Medicine, Zhejiang Provincial People’s Hospital,People’s Hospital of Hangzhou Medical Collage, Hangzhou, Zhejiang, 310014, China
- ⁴Zhejiang Provincial Center for Disease Control and Prevention, 630 Xincheng Road, Hangzhou, Zhejiang, 310051, China

Received: November 20, 2018; Published: November 30, 2018

DOI: 10.26717/BJSTR.2018.11.002126

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Abstract

Aqueous and ethanol extracts from the mycelia of Cordyceps Ophioglossoides have been used as a nutritional supplement, especially for women suffering from massive postpartum vaginal bleeding in Southwest China. However, there is limited information regarding its components, extraction conditions and bioactivities. The present study was undertaken to identify total phenol and flavonoid content of cultured mycelia of Cordyceps Ophioglossoides under optimized extraction condition. The anti-oxidative activities of two extracts were evaluated in three in vitro systems including 1, 1-diphenyl-2-picrylhydracyl (DPPH) radical scavenging assay, superoxide radical scavenging assay, and hydroxyl radical scavenging assay. The estrogenic activity of the 20% ethanol extracts (20%EE) was studied by cell proliferation assay, by luciferase reporter assay and by measuring expression of ER endogenous target genes in human breast cancer cells. These results suggest that the extracts of Cordyceps Ophioglossoides have high levels of phenols and flavonoids as well as anti-oxidative and estrogenic bioactivities.

Keywords : Cordyceps Ophioglossoides; Phenol; Flavonoid; Aqueous and Ethanol Extracts; Anti-Oxidative Activity; Estrogenic Activity

Abbreviations : EE: Ethanol Extracts; SERM: Selective Estrogen Receptor Modulators; DPPH: Diphenyl-2-Picrylhydracyl; TBHQ: Tert-Butylhydroquinone; BHT: Butyl-4-Methylphenol; E2: Estradiol; TPC: Total Phenols Content; TFC: Total Flavonoid Content; AE: Aqueous Extracts MTT, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyl Tetrazolium Bromide; ROS: Reactive Oxygen Species