Mini Review
Biological systems have magnetic properties due to microscopic
atomic dipoles in their compounds which align themselves
individually if a magnetic field is applied. As a result, a biological
system can align along the direction of an applied magnetic field
following the theory of paramagnetism. This effect can be induced
also in simple organic systems. For instance, the α-helix is the
main structure of a protein. We should not be surprised by this
effect as proteins α-helix has generally a relevant dipole moment
which allows possible orientation of a protein along the direction
of an applied electromagnetic field. For instance, hemoglobin has
a dipole moment of 280 D [1]. Instead, what makes us wonder is
the fact that this effect occurs even at very small intensity of an
electromagnetic field regardless of its frequency. In order that this
effect can be highlighted a sophisticated technique should be used,
that is Fourier Transform Infrared (FTIR) spectroscopy.
FTIR spectroscopy can be considered as a valuable tool for
analyzing the structure of proteins or other simple organic systems
in aqueous solutions [2,3]. The use of this technique has evidenced
a significant increase in intensity of proteins Amide I and Amide
II vibration bands after exposure of typical proteins in water
solutions to an electromagnetic field at a low intensity at 100mW/
m2 in the range 0.9 – 2.5 GHz [4-7]. These vibration bands are
characteristic of proteins secondary structure and are due above all
to the α-helix content. Hence, an increasing of Amide I and II bands
can be explained assuming that proteins α-helix aligned along the
direction of applied electromagnetic field inducing an increasing of total amount of dipole moment. Typical proteins in bidistilled
water solution were used in these experiments [4-6] in order to
schematize cellular environment in which they are embedded. This
effect was also observed in typical human cells [8-13]. Even this
result should not surprise us because proteins α-helices are present
in all types of cellular membrane channels [14-16]. In particular,
cellular membrane protein accounting for about 50% of its mass
forming the wall of cells channels [17].
Rotation induced by exposure to magnetic fields was also
observed in DNA and chromosomes in neuronal-like cells. Indeed,
significant decrease in intensity of the phosphate bands in the DNA
infrared region was observed by FTIR spectroscopy after exposure
of human neuronal-like cells to static and 50Hz magnetic field at
the low-intensity value of 1mT [18]. This result can be explained
assuming that uncoiling and unpackaging of chromatin constituents
in chromosomes occurred after exposure, due to the torque induced
by the applied magnetic field and the consequent chromosome
alignment towards the direction of the field. Incidentally, the
rotation of chromosomes produced by exposure to a magnetic
field was observed directly by microscope of some plants, Allium
sativum and Vicia faba, exposed to a magnetic field [18]. Also, this
result depends on paramagnetic property of DNA constituents.
However, these results seem that cannot be explained theoretically.
In fact, Adair showed that the torque of an electromagnetic field at
high intensity is lower than the mean angular momentum due to
thermal molecular agitation in organic matter [19].
Nevertheless, it was shown that the impulse from an
electromagnetic field integrated over an interval of some hour will
overhead the mean angular momentum from thermal agitation
[20], demonstrating that the torque induced by high frequency
electromagnetic field from wireless devices can affect organic
matter due to the duration of exposure. Indeed, the simulation of
Adair can be applied to a macrodipole exposed to an electromagnetic
field provided that the viscous damper of the medium in which it is
embedded can be neglected, for instance in the vacuum. In contrast,
organic systems are embedded in aqueous solutions with viscous
damper that cannot be neglected. As a result, the macrodipole
cannot follow the oscillation of the electromagnetic field due to the
inertia of molecules and the viscosity of the medium, so that it will
place itself at an average position along the direction of the field
[7]. Hence, the entire exposure time should be taken into account in
the calculation of the torque induced by an applied electromagnetic
field so that the simulation is in agreement with experimental
results.
References
- Antosiewicz J, Porschke D (1995) Electrostatics of hemoglobins from measurements of the electric dichroism and computer simulations. Biophys J 68(2): 655-664.
- Byler DM, Susi H (1986) Examination of the secondary structure of proteins by deconvolved FTIR spectra. Biopolymer 25(3): 469-487.
- Surewicz WK, Mantsch HH (1988) New insight into protein secondary structure from resolution-enhanced infrared spectra. Biochim Biophys Acta 952(2): 115-130.
- Calabrò E, Magazù S (2016) Parallel β-sheet Vibration Band Increases with Proteins Dipole Moment under Exposure to 1765 MHz Microwaves. Bioelectromagnetics 37(2): 99-107.
- Calabrò E, Magazù S (2017) The α-Helix Alignment of Proteins in Water Solution towards a High Frequency Electromagnetic Field: a FTIR Spectroscopy Study. Electromagnetic Biology and Medicine 36(3): 279-288.
- Calabrò E, Magazù S, Campo S (2012) Microwave-induced increase of amide I and amide II vibration bands and modulating functions of sodium-chloride, sucrose and trehalose aqueous solutions: The case study of Haemoglobin. Research Journal of Chemistry and Environment 16(4): 59-67.
- Calabrò E, Magazù S (2018) Resonant interaction between electromagnetic fields and proteins: A possible starting point for the treatment of cancer. Electromagnetic Biology and Medicine 37(3): 155-168.
- Calabrò E, Condello S, Currò M, Ferlazzo N, Vecchio M, et al. (2013) 50 Hz Electromagnetic Field Produced Changes in FTIR Spectroscopy Associated with Mitochondrial Transmembrane Potential Reduction in Neuronal-Like SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity 2013: 414393.
- Calabrò E (2016) Competition between Hydrogen Bonding and Protein Aggregation in Neuronal-Like Cells under Exposure to 50 Hz Magnetic Field. International Journal of Radiation Biology 92(7): 395-403.
- Calabrò E, Magazù S (2019) New Perspectives in the Treatment of Tumor Cells by Electromagnetic Radiation at Resonance Frequencies in Cellular Membrane Channels. The Open Biotechnology Journal 13: 105-110.
- Calabrò E, Magazù S (2019) Infrared Spectroscopic Demonstration of Magnetic Orientation in SH-SY5Y Neuronal-Like Cells Induced by Static or 50 Hz Magnetic Fields. International Journal of Radiation Biology 95(6): 781-787.
- Calabrò E, Currò M, Caccamo MT (2020) Competition between N-H bending vibration and α-helix polarization under 50 Hz magnetic field in SH-SY5Y neuronal-like cells. Spectroscopy Letters: An International Journal for Rapid Communication 53(6): 458-465.
- Calabrò E, Magazù S (2020) Applying Resonant Electromagnetic Fields in Membrane Channels of Tumor Cells: The Future of Cancer Treatment. Biomed J Sci & Tech Res 31(4): 2020.
- Becker L, Bannwarth M, Meisinger C (2005) Preprotein translocase of the outer mitochondrial membrane: Reconstituted Tom 40 forms a characteristic TOM pore. J Mol Biol 353: 1011-1020.
- Feria Bourrellier AB, Valot B, Guillot A (2010) Chloroplast acetyl-CoA carboxylase activity is 2-oxoglutarate-regulated by interaction of PII with the biotin carboxyl carrier subunit. Proc Natl Acad Sci USA 107: 502-507.
- Szabo I, Zoratti M (2014) Mitochondrial channels: Ion fluxes and more. Physiol Rev 94: 519-608.
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- Calabrò E, Goswami HK, Magazù S (2020) Chromosome aberration in typical biological systems under exposure to low- and high intensity magnetic fields. Electromagnetic Biology and Medicine 39(2): 97-108.
- Adair RK (2000) Biophysics Limits on the Biological Effects of Ultrawide band Electromagnetic Radiation. Springer.
- Calabrò E, Magazù S (2018) Direct spectroscopic evidence for competition between thermal molecular agitation and magnetic field in a tetrameric protein in aqueous solution. Physics Letters A 382: 1389-1394.