*Corresponding author:
Michał J Markuszewski, Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al Gen J Hallera 107, 80-416 Gdańsk, PolanReceived: March 16, 2018; Published: April 03, 2018
DOI: 10.26717/BJSTR.2018.03.000906
To view the Full Article Peer-reviewed Article PDF
The aim of the study was to develop and optimize a simple, fast and applicable method for the quantitative determination of methylene blue. Different parameters, such as composition of separation buffer, pH value and injection time were investigated in order to obtain the best peak shape and reproducibility within the shortest analysis time. The method developed demonstrated linear response over the range of 20- 100 μg/ml. Micellar electrokinetic chromatographic assay proposed is favorable in terms of the overall analysis time and method simplicity.
Keywords:Methylene Blue; Micellar Electrokinetic Chromatography; Method Development
Abbreviations:MB: Methylene Blue; CE: Capillary Electrophoresis; LC: Liquid Chromatography; MS: Mass Spectrometry; DAD: Diode Array Detection; UV-VIS: Ultraviolet-Visible Spectroscopy; SDS: Sodium Dodecyl Sulfate; BGE: Background Electrolyte; MECK: Micellar Electrokinetic Chromatography; CZE: Capillary Zone Electrophoresis; CPA: Corrected Peak Areas
Abstract| Introduction| Experimental| Experimental| Discussion| Conclusion| References|